Acute toxicity of total ammonia to Macrobrachium rosenbergii postlarvae at different salinity levels.

Nitrogen compounds, particularly ammonium, nitrite and nitrate, are a major problem in shrimp production systems. These compounds can accumulate in the aquatic environment and reach harmful or even lethal levels. Thus, monitoring the levels of nitrogenous compounds such as ammonia and studying their effects on the animals are essential. One tool used for this purpose is acute toxicity testing based on the evaluation of LC50 values. Furthermore, tools that can help improve the performance of aquatic organisms in culture are needed. The present study aimed to evaluate the effect of salinity on the toxicity of total ammonia to postlarvae of the freshwater prawn Macrobrachium rosenbergii. For this purpose, acute toxicity testing (LC50-96h) was performed using 540 postlarvae with a mean weight of 0.13 g and a mean total length of 2.47 cm, divided into 54 experimental units of two liters each. A completely randomized design in a 3×6 factorial scheme was used, combining three salinities (0, 5, and 10 g.L-1) and six total ammonia concentrations (0, 8, 16, 32, 64, and 128 mg.L-1), with three replicates per combination. The LC50 values for M. rosenbergii postlarvae at 24, 48, 72, and 96 h and their respective confidence intervals (95%) were estimated using the trimmed Spearman-Karber method. The results showed that salinities of 5 or 10 g.L-1 did not reduce the acute toxicity of total ammonia.

Topological phases of graphene-Kagome systems.

The growing skill in the synthesis processes of new materials has intensified the interest in exploring the properties of systems modeled by more complex lattices. Two-dimensional super-honeycomb lattices, have been investigated in metallic organic frameworks. They turned out as a significant route to the emergence of localized electronic responses manifested as flat bands in their structure with topological isolating behavior. A natural inquiry is a complete analysis of their topological phases in the presence of electronic correlation effects. Here we analyze the electron-electron correlation effects via Hubbard mean-field approximation on the topological phases of 2D and quasi-1D graphene-Kagome lattices. The 2D spin conductivity phase's diagrams describe metallic, trivial, and topological insulating behaviors, considering different energy coupling and electronic occupations. Our results pave the way to smart-engineered nanostructured devices with relevant applications in spintronics and transport responses.

Ovarian development of Xiphopenaeus kroyeri (Crustacea: Penaeidae) from Espírito Santo, southeastern Brazil.

This study aimed to describe and characterize the stages of gonadal development of females of Xiphopenaeus kroyeri caught by artisanal fishers in Espírito Santo state, southeastern region of Brazil. All females (n= 1,831) were subjected to macroscopic and microscopic morphological analysis (n= 333) of the ovaries. From the morphology, coloration and degree of turgidity of the fresh ovary, macroscopic analysis determined five stages of gonadal development. The macroscopic analysis showed difficulties in differentiating the immature and spawning stages due to the similarity between the colors of the ovaries, which confirms the need to perform the macroscopic and histological analysis simultaneously for fisheries management studies. Microscopic observations allowed us to analyze the following six stages of cell development: oogonia, previtellogenic oocytes, primary vitellogenic oocytes, secondary vitellogenic oocytes, mature oocytes and atretic oocytes. From this, five stages of gonadal development were defined, i.e., immature, early development, advanced development, mature and spawned. The presence of peripheral bodies was not observed in this species. These results help to clarify and better understand the reproductive and population aspects of the Atlantic Seabob, which are fundamental for the establishment of management and conservation measures of this resource.

Zootechnical indices and digestibility in juveniles of tambaqui Colossoma macropomum fed a diet containing particulate maize / Índices zootécnicos e digestibilidade em juvenis de tambaqui Colossoma macropomum alimentados com dieta contendo milho particulado

Three experiments were done with different particle sizes of corn feed on its zootechnical performance, passing rate and apparent digestibility of juvenile tambaqui (Colossoma macropomum). In the first, 200 juveniles were used and distributed in 20 tanks (220 L), 10 fish per unit (four replicates). The experimental system used to record passage time was composed of five incubators with 200 L. In the second, 75 juveniles were used per 55 days (three replications). In the third, 75 juveniles were used and distributed in five incubators of 200 L. All experiments were performed randomly. Different linear behavior treatments were observed for apparent digestibility of crude protein; and the smaller particle size (150 µm) had better results digestibility. smaller particle size of the corn had better results, affected the growth performance of tambaqui and the apparent digestibility of crude protein and ether extract. Thus, is recommended that a particle size of 150 µm of corn be used for tambaqui.

Três experimentos foram realizados com diferentes tamanhos de partícula de ração para o desempenho zootécnico, taxa de aprovação e digestibilidade aparente de tambaqui juvenil (Colossoma macropomum). No primeiro, foram utilizados 200 juvenis e distribuídos em 20 tanques (220 L), 10 peixes por unidade (quatro repetições). O sistema experimental utilizado para registrar o tempo de passagem foi composto por cinco incubadoras com 200 L. No segundo, 75 juvenis foram utilizados por 55 dias (três repetições). No terceiro, 75 juvenis foram utilizados e distribuídos em cinco incubadoras de 200 L. Todos os experimentos foram realizados aleatoriamente. Diferentes tratamentos de comportamento linear foram observados para digestibilidade aparente da proteína bruta; e o menor tamanho de partícula (150 µm) apresentou melhor digestibilidade dos resultados. menor tamanho de partícula do milho apresentou melhores resultados, afetou o desempenho de crescimento de tambaquis e a digestibilidade aparente da proteína bruta e extrato etéreo. Portanto, recomenda-se o uso de um tamanho de partícula de 150 µm de milho para o tambaqui.

Zootechnical indices and digestibility in juveniles of tambaqui Colossoma macropomum fed a diet containing particulate maize / Índices zootécnicos e digestibilidade em juvenis de tambaqui Colossoma macropomum alimentados com dieta contendo milho particulado

Three experiments were done with different particle sizes of corn feed on its zootechnical performance, passing rate and apparent digestibility of juvenile tambaqui (Colossoma macropomum). In the first, 200 juveniles were used and distributed in 20 tanks (220 L), 10 fish per unit (four replicates). The experimental system used to record passage time was composed of five incubators with 200 L. In the second, 75 juveniles were used per 55 days (three replications). In the third, 75 juveniles were used and distributed in five incubators of 200 L. All experiments were performed randomly. Different linear behavior treatments were observed for apparent digestibility of crude protein; and the smaller particle size (150 μm) had better results digestibility. Smaller particle size of the corn had better results, affected the growth performance of tambaqui and the apparent digestibility of crude protein and ether extract. Thus, is recommended that a particle size of 150 μm of corn be used for tambaqui.

Três experimentos foram realizados com diferentes tamanhos de partícula de ração para o desempenho zootécnico, taxa de aprovação e digestibilidade aparente de tambaqui juvenil (Colossoma macropomum). No primeiro, foram utilizados 200 juvenis e distribuídos em 20 tanques (220 L), 10 peixes por unidade (quatro repetições). O sistema experimental utilizado para registrar o tempo de passagem foi composto por cinco incubadoras com 200 L. No segundo, 75 juvenis foram utilizados por 55 dias (três repetições). No terceiro, 75 juvenis foram utilizados e distribuídos em cinco incubadoras de 200 L. Todos os experimentos foram realizados aleatoriamente. Diferentes tratamentos de comportamento linear foram observados para digestibilidade aparente da proteína bruta; e o menor tamanho de partícula (150 μm) apresentou melhor digestibilidade dos resultados. menor tamanho de partícula do milho apresentou melhores resultados, afetou o desempenho de crescimento de tambaquis e a digestibilidade aparente da proteína bruta e extrato etéreo. Portanto, recomenda-se o uso de um tamanho de partícula de 150 μm de milho para o tambaqui.

Zootechnical indices and digestibility in juveniles of tambaqui Colossoma macropomum fed a diet containing particulate maize

Abstract Three experiments were done with different particle sizes of corn feed on its zootechnical performance, passing rate and apparent digestibility of juvenile tambaqui (Colossoma macropomum). In the first, 200 juveniles were used and distributed in 20 tanks (220 L), 10 fish per unit (four replicates). The experimental system used to record passage time was composed of five incubators with 200 L. In the second, 75 juveniles were used per 55 days (three replications). In the third, 75 juveniles were used and distributed in five incubators of 200 L. All experiments were performed randomly. Different linear behavior treatments were observed for apparent digestibility of crude protein; and the smaller particle size (150 m) had better results digestibility. smaller particle size of the corn had better results, affected the growth performance of tambaqui and the apparent digestibility of crude protein and ether extract. Thus, is recommended that a particle size of 150 m of corn be used for tambaqui.

Resumo Três experimentos foram realizados com diferentes tamanhos de partícula de ração para o desempenho zootécnico, taxa de aprovação e digestibilidade aparente de tambaqui juvenil (Colossoma macropomum). No primeiro, foram utilizados 200 juvenis e distribuídos em 20 tanques (220 L), 10 peixes por unidade (quatro repetições). O sistema experimental utilizado para registrar o tempo de passagem foi composto por cinco incubadoras com 200 L. No segundo, 75 juvenis foram utilizados por 55 dias (três repetições). No terceiro, 75 juvenis foram utilizados e distribuídos em cinco incubadoras de 200 L. Todos os experimentos foram realizados aleatoriamente. Diferentes tratamentos de comportamento linear foram observados para digestibilidade aparente da proteína bruta; e o menor tamanho de partícula (150 m) apresentou melhor digestibilidade dos resultados. menor tamanho de partícula do milho apresentou melhores resultados, afetou o desempenho de crescimento de tambaquis e a digestibilidade aparente da proteína bruta e extrato etéreo. Portanto, recomenda-se o uso de um tamanho de partícula de 150 m de milho para o tambaqui.

Growth and hematological responses of tambaqui, Colossoma macropomum fed different levels of rice, Oryza spp / Crescimento e resposta hematológica do tambaqui, Colossoma macropomum alimentados com diferentes níveis de arroz, Oryza spp

Abstract The tambaqui (Colossoma macropomum) is a fish of primary importance in South American aquaculture, principally in the Amazon region and has a particularly unique diet in the wild. Oryza spp, or Wild rice as it is commonly known, is native to the floodplains of muddy rivers in the Amazon region. The aim of this study was to analyze the physical performance and the effects of dietary levels of Oryza spp. via the hematological parameters, total food intake, conversion efficiency, growth ratio and swimming performance of this fish. Diets containing 45% Oryza spp. induced the best performance in tambaqui. Diets containing 15% and 30% did not affect these indices, thus indicating that this amount of Oryza can be used as an alternative energy source for this important species within Brazilian aquaculture.

Resumo O tambaqui Colossoma macropomum é um peixe de importância na aquicultura brasileira, especialmente na região amazônica. O arroz silvestre é comum nas planícies inundadas dos rios de aguas barrentas da Amazônia. O objetivo deste trabalho foi analisar os efeitos combinados do treinamento físico e dos níveis dietéticos de Oryza spp. nos parâmetros hematológicos, consumo de alimentos, eficiência de conversão, taxa de crescimento e desempenho de natação deste peixe. Dietas contendo 45% de arroz apresentaram o melhor desempenho para tambaquis. As dietas contendo 15% e 30% não afetaram esses índices, indicando que alguma quantidade de arroz pode ser usada como fonte de energia alternativa para esta importante espécie dentro da aquicultura.

Zootechnical indices and digestibility in juveniles of tambaqui Colossoma macropomum fed a diet containing particulate maize.

Three experiments were done with different particle sizes of corn feed on its zootechnical performance, passing rate and apparent digestibility of juvenile tambaqui (Colossoma macropomum). In the first, 200 juveniles were used and distributed in 20 tanks (220 L), 10 fish per unit (four replicates). The experimental system used to record passage time was composed of five incubators with 200 L. In the second, 75 juveniles were used per 55 days (three replications). In the third, 75 juveniles were used and distributed in five incubators of 200 L. All experiments were performed randomly. Different linear behavior treatments were observed for apparent digestibility of crude protein; and the smaller particle size (150 µm) had better results digestibility. smaller particle size of the corn had better results, affected the growth performance of tambaqui and the apparent digestibility of crude protein and ether extract. Thus, is recommended that a particle size of 150 µm of corn be used for tambaqui.

B-mode and Doppler ultrasonography of abdominal structures of the peccary (Tayassu tajacu, Linnaeus 1758) / [Ultrassonografias Doppler e modo-B das estruturas abdominais de catetos (Tayassu tajacu Linnaeus, 1758)]

This study used B-mode and Doppler ultrasonography to characterize the abdominal structures of healthy peccaries raised in captivity. Fifteen peccaries were used for this study. The urinary vesicle appeared as an ovoid structure, located in the abdominal and pelvic transition, with a hyperechogenic, thin, smooth, and regular wall. The kidneys presented retroperitoneal topography and had similar sizes. The kidney/aorta ratio had an average value of 10.53±15cm (right) and 10.23±0.12 (left). The right adrenal gland had a length of 1.93±0.34cm and diameter of 0.56±0.16cm. The left adrenal gland had a length of 1.85±0.42cm and diameter of 0.52±0.11cm. The spleen had a diameter of 1.13±0.18cm. The hepatic vein demonstrated polyphasic flow in pulsed Doppler, with two retrograde peaks and an anterograde peak with a flow velocity of 25.7±0.83cm/s. The abdominal aorta had a diameter of 0.58±0.05cm and a flow velocity of 115.17±5.32cm/s. The morphological and hemodynamic study of the abdominal structures of the peccary, observed through B-mode and Doppler ultrasonography, aided in identifying the size, shape, position, echogenicity, and echotexture of the abdominal organs and in making inferences about the normal parameters for these structures in this species.(AU)

Este estudo teve como objetivo utilizar as ultrassonografias de modo-B e Doppler para caracterizar as estruturas abdominais de um cateto sadio criado em cativeiro. Quinze catetos foram utilizados para este estudo. A vesícula urinária apareceu como uma estrutura ovoide, localizada na transição entre as partes abdominal e pélvica, com uma parede hiperecogênica, fina, lisa e regular. Os rins apresentaram topografia retroperitoneal e tamanhos semelhantes. A relação rim/aorta teve um valor médio de 10,53 ± 15cm (direita) e 10,23 ± 0,12cm (esquerda). A glândula adrenal direita tinha um comprimento de 1,93 ± 0,34cm e um diâmetro de 0,56 ± 0,16cm. A glândula suprarrenal esquerda tinha um comprimento de 1,85 ± 0,42cm e um diâmetro de 0,52 ± 0,11cm. O baço tinha um diâmetro de 1,13 ± 0,18cm. A veia hepática demonstrou fluxo polifásico no Doppler pulsátil, com dois picos retrógrados e um pico anterógrado com velocidade de fluxo de 25,7±0,83cm/s. A aorta abdominal tinha um diâmetro de 0,58 ± 0,05cm e uma velocidade de fluxo de 115,17±5,32cm/s. Os estudos morfológico e hemodinâmico das estruturas abdominais do queixada, observadas por meio das ultrassonografias modo-B e Doppler, auxiliaram na identificação do tamanho, da forma, da posição, da ecogenicidade e da ecotextura dos órgãos abdominais e na realização de inferências sobre os parâmetros de normalidade para as estruturas nas espécies.(AU)

Growth and hematological responses of tambaqui, Colossoma macropomum fed different levels of rice, Oryza spp.

The tambaqui (Colossoma macropomum) is a fish of primary importance in South American aquaculture, principally in the Amazon region and has a particularly unique diet in the wild. Oryza spp, or Wild rice as it is commonly known, is native to the floodplains of muddy rivers in the Amazon region. The aim of this study was to analyze the physical performance and the effects of dietary levels of Oryza spp. via the hematological parameters, total food intake, conversion efficiency, growth ratio and swimming performance of this fish. Diets containing 45% Oryza spp. induced the best performance in tambaqui. Diets containing 15% and 30% did not affect these indices, thus indicating that this amount of Oryza can be used as an alternative energy source for this important species within Brazilian aquaculture.

Ornamental fishing in the region of Barcelos, Amazonas: socioeconomic description and scenario of activity in the view of piabeiros / A pesca ornamental na região de Barcelos, Amazonas: descrição socieconômica e cenário da atividade na visão dos piabeiros

Abstract The Negro river basin is considered the largest area of extractive of ornamental fish in Brazil. This area has fundamental importance for the populations from the Amazon. The present study aimed to describe socioeconomic profile of ornamental fishermen known as "piabeiros" in the Municipality of Barcelos, as well as the ornamental fishery, fisheries area, target species, environments, fishing techniques, equipment, capture techniques and main difficulties faced by the current activity. This study was carried out in municipality of Barcelos, through semi - structured interviews, with artisanal ornamental fishermen (N= 89). The main families of ornamental fish caught and traded were: Characidae, Lebiasinidae, Gasteropelecidae, Cichilidae, Anostomidae, Loricaridae, Potamotrygonidae and Gymnotidae. The main catchment areas were igarapés, lakes, flooded fields, beaches, river banks and igapó forest. Rapiché was the most used equipment in the fisheries both by the fishermen of the urban areas (43.81%) and rural (41.89%). Most of the fishermen are associated with the colony of fishermen of Barcelos (Z33). The data showed that the dynamics of ornamental fishing have changed in a short time and directly affected fishermen, in addition to the low age renewal with the participation of younger fishermen, threatening the transmission of ecological knowledge to future generations. As a result, the increase of the problems related to the productive chain and absence of public power to the activity, since ornamental fishing has already been treated as one of the main economic activities more important for the local communities and for the State of Amazonas.

Resumo A bacia do rio Negro é considerada a maior área de extrativismo de peixes ornamentais do Brasil. Esta área possui importância fundamental para as populações da Amazônia. O presente estudo objetivou descrever o perfil socioeconômico de pescadores conhecidos como "piabeiros" no município de Barcelos, bem como a pesca ornamental, área de pesca, espécies-alvo, ambientes, técnicas de pesca, equipamentos, técnicas de captura e dificuldades enfrentadas nesta atividade. O estudo foi realizado por meio de entrevistas semi - estruturadas, com pescadores artesanais ornamentais (N= 89). Os dados mostram que as famílias de peixes ornamentais capturados e comercializados foram: Characidae, Lebiasinidae, Gasteropelecidae, Cichilidae, Anostomidae, Loricaridae, Potamotrygonidae e Gymnotidae. As áreas de captura descritas foram igarapés, lagos, alagados, praias, margens de rios e floresta de igapó. O Rapiché foi o equipamento mais utilizado nas pescarias tanto pelos pescadores das áreas urbanas (43,81%) como rurais (41,89%). A maioria dos pescadores está associada à colônia de pescadores de Barcelos (Z33). Os dados coletados mostraram que a dinâmica da pesca ornamental mudou em pouco tempo e afetou diretamente os pescadores. Além da baixa taxa de renovação, com a participação de pescadores mais jovens, ameaçando a transmissão de conhecimento ecológico para as gerações futuras. Como resultado, notamos o aumento dos problemas relacionados à cadeia produtiva e a ausência de poder público na atividade. A pesca ornamental já foi tratada como uma das principais atividades econômicas para as comunidades locais e para o Estado do Amazonas.

Reaching task performance is associated to neuromuscular junction adaptations in rats with induced diabetes mellitus.

Upper limb performance is affected by diabetes mellitus (DM). Neuromuscular junction (NMJ) is a key structure to understand the relationship between performance and morphology in DM. The aim of the study was to analyze NMJ plasticity due to DM in an animal model and its relationship with the function of forelimbs in rats. Twelve Wistar rats were divided into control (C) and DM groups. Animals were trained to perform a grasping task, following procedures of habituation, shaping, and reaching task. DM was induced using streptozotocin. Forelimb neuromuscular performance for dexterity was evaluated one day before DM induction and five weeks following induction. After that, biceps, triceps, and finger flexors and extensors were removed. Connective tissue and muscle fiber cross-sectional area (CSA) were measured. NMJ was assessed by its morphometric characteristics (area, perimeter, and maximum diameter), using ImageJ software. Motor performance analyses were made using single pellet retrieval task performance test. Student's t-test was used for comparisons between groups. A significant decrease in all NMJ morphometric parameters was observed in the DM group compared with the C group. Results showed that DM generated NMJ retraction in muscles involved in a reaching task. These alterations are related to signs of muscular atrophy and to poor reaching task performance. In conclusion, induced DM caused NMJ retraction and muscular atrophy in muscles involved in reaching task performance. Induced DM caused significantly lower motor performance, especially in the final moments of evaluation, when DM compromised the tropism of the muscular tissue.

Ornamental fishing in the region of Barcelos, Amazonas: socioeconomic description and scenario of activity in the view of "piabeiros".

The Negro river basin is considered the largest area of extractive of ornamental fish in Brazil. This area has fundamental importance for the populations from the Amazon. The present study aimed to describe socioeconomic profile of ornamental fishermen known as "piabeiros" in the Municipality of Barcelos, as well as the ornamental fishery, fisheries area, target species, environments, fishing techniques, equipment, capture techniques and main difficulties faced by the current activity. This study was carried out in municipality of Barcelos, through semi - structured interviews, with artisanal ornamental fishermen (N= 89). The main families of ornamental fish caught and traded were: Characidae, Lebiasinidae, Gasteropelecidae, Cichilidae, Anostomidae, Loricaridae, Potamotrygonidae and Gymnotidae. The main catchment areas were igarapés, lakes, flooded fields, beaches, river banks and igapó forest. Rapiché was the most used equipment in the fisheries both by the fishermen of the urban areas (43.81%) and rural (41.89%). Most of the fishermen are associated with the colony of fishermen of Barcelos (Z33). The data showed that the dynamics of ornamental fishing have changed in a short time and directly affected fishermen, in addition to the low age renewal with the participation of younger fishermen, threatening the transmission of ecological knowledge to future generations. As a result, the increase of the problems related to the productive chain and absence of public power to the activity, since ornamental fishing has already been treated as one of the main economic activities more important for the local communities and for the State of Amazonas.

Reaching task performance is associated to neuromuscular junction adaptations in rats with induced diabetes mellitus

Upper limb performance is affected by diabetes mellitus (DM). Neuromuscular junction (NMJ) is a key structure to understand the relationship between performance and morphology in DM. The aim of the study was to analyze NMJ plasticity due to DM in an animal model and its relationship with the function of forelimbs in rats. Twelve Wistar rats were divided into control (C) and DM groups. Animals were trained to perform a grasping task, following procedures of habituation, shaping, and reaching task. DM was induced using streptozotocin. Forelimb neuromuscular performance for dexterity was evaluated one day before DM induction and five weeks following induction. After that, biceps, triceps, and finger flexors and extensors were removed. Connective tissue and muscle fiber cross-sectional area (CSA) were measured. NMJ was assessed by its morphometric characteristics (area, perimeter, and maximum diameter), using ImageJ software. Motor performance analyses were made using single pellet retrieval task performance test. Student's t-test was used for comparisons between groups. A significant decrease in all NMJ morphometric parameters was observed in the DM group compared with the C group. Results showed that DM generated NMJ retraction in muscles involved in a reaching task. These alterations are related to signs of muscular atrophy and to poor reaching task performance. In conclusion, induced DM caused NMJ retraction and muscular atrophy in muscles involved in reaching task performance. Induced DM caused significantly lower motor performance, especially in the final moments of evaluation, when DM compromised the tropism of the muscular tissue.

Echocardiographic reference ranges for sedated healthy peccaries ( Tayassu tajacu, Linnaeus, 1758 ) / Valores de referência para catetos saudáveis sedados

Peccaries are wild mammals belonging to the Tayassu genus that are found almost everywhere in the Americas and have demonstrated great potential as an experimental model for scientific investigations. Twelve healthy adult animals were sedated to perform echocardiographic examinations in B, M and Doppler mode. The variables that exhibited statistically significant correlation coefficients with weight were LVFWd, LVIDd, LVIDs, E wave, A'RV, MAM, and TAPSE. The HR exhibited a negative relationship with the IVRT. The LA variable showed a positive correlation with the AO. The MAM exhibited correlations with the LVIDd and LVIDs. The TAPSE showed positive correlations with the E'RV and A'RV. The present study provides the first reference values for echocardiographic measurements in B, M and Doppler modes from peccaries anesthetized with ketamine and midazolam. Echocardiography was easy to perform in collared peccaries, and the collected data revealed values that can aid in their clinical management and conservation.(AU)

Catetos são mamíferos selvagens, pertencentes ao gênero Tayassu, encontrados em quase toda a América, os quais têm se destacado como modelos experimentais. Doze animais adultos saudáveis foram sedados para a realização de exames ecocardiográficos em modos B, M e Doppler. As variáveis que apresentaram coeficientes de correlação estatisticamente significativos em relação ao peso foram: LVFWd, LVIDd, LVIDs, onda E, A'RV, MAM, TAPSE. O HR mostrou correlação positiva com LA/AO, onda E, onda A, MAM, TAPSE e E/IVRT, e negativa com IVRT. Os valores de AVmax mostraram correlações positivas com onda E e onda A'. A variável LA mostrou correlação positiva com AO e correlação negativa com IVSd. A EF apresentou forte correlação com a relação de ondas FS, E/A, com A', E'RV. FS apresentou correlação positiva com a relação de onda E/A. O MAM mostrou correlação com LVIDd e LVIDs. TAPSE mostrou correlações positivas com E'RV e A'RV. O presente estudo forneceu os primeiros valores de referência para medições ecocardiográficas em modos B, M e Doppler de catetos anestesiados com ketamina e midazolam. O exame ecocardiográfico em catetos foi de fácil execução e os dados encontrados evidenciaram valores que podem auxiliar no seu manejo clínico e conservação.(AU)

Influence of parasite load on the diagnosis and occurrence of eosinophilia in alcoholic patients infected with Strongyloides stercoralis.

Alcoholic patients are more susceptible to Strongyloides stercoralis infection. The chronic use of alcohol raises the levels of endogenous corticosteroids, which regulates the development of larvae and stimulates the differentiation of rhabditiform into infective filariform larvae, thus inducing internal autoinfection. Therefore, early diagnosis is important to prevent severe strongyloidiasis. The aim of this study was to evaluate the efficacy of parasitological methods, according to the parasite load and the number of stool samples, for diagnosis of S. stercoralis infection, as well the peripheral blood eosinophil count in alcoholic patients. A total of 330 patients were included in this study. The diagnosis was established using three parasitological methods: agar plate culture, Baermann-Moraes method and spontaneous sedimentation. Peripheral eosinophilia was considered when the level was >600 eosinophils/mm3. The agar plate culture (APC) had the highest sensitivity (97.3%). However, the analysis of multiple samples increased the sensitivity of all parasitological methods. The sensitivities of the methods were influenced by the parasite load. When the larval number was above 10, the sensitivity of APC was 100%, while in spontaneous sedimentation the sensitivity reached 100% when the larval number was above 50. In the present study, 15.4% of alcoholic patients infected with S. stercoralis (12/78) had increased peripheral blood eosinophil count (above 600 eosinophils/mm3). For an efficient parasitological diagnosis of S. stercoralis infection in alcoholic patients, repeated examination by two parasitological methods must be recommended, including agar plate culture due to its higher sensitivity. Moreover, S. stercoralis infection was associated with eosinophilia, mostly in patients excreting up to 10 larvae/g faeces.

Insulinoma: A retrospective study analyzing the differences between benign and malignant tumors.

BACKGROUND/OBJECTIVES: Insulinoma is a rare pancreatic tumor and, usually, a benign disease but can be a malignant one and, sometimes, a highly aggressive disease. The aim of this study was to determine differences between benign and malignant tumors. METHODS: Retrospective study of 103 patients with insulinoma treated in a tertiary center. It was analyzed demographic, clinical, laboratory, localization and histologic analysis of tumor and follow up data of subjects in order to identify differences between individuals benign and malignant disease. RESULTS: Almost all patients (87%) had a benign tumor and survival rates of 100% following pancreatic tumor surgery. Those with malignant tumors (13%) have a poor prognosis, 77% insulinoma-related deaths over a period of 1-300 months after the diagnosis with a survival rate of 24% in five years. The following factors are associated with an increased risk of malignant disease: duration of symptoms < 24 months, fasting time for the occurrence of hypoglycemia < 8 h, blood plasma insulin concentration ≥ 28 µU/mL and C-peptide ≥ 4.0 ng/mL at the glycemic nadir and tumor size ≥ 2.5 cm. CONCLUSIONS: Our data help to base the literature about these tumors, reinforcing that although insulinoma is usually a single benign and surgically treated neoplasia, the malignant one is difficult to treat. We highlight the data that help predict a malignancy behavior of tumor and suggest a long follow up after diagnosis in these cases.

An individualised risk-adapted protocol of pre- and post transplant zoledronic acid reduces bone loss after allogeneic stem cell transplantation: results of a phase II prospective trial.

Bone loss occurs frequently following allogeneic haematopoietic stem cell transplantation (alloSCT). The Australasian Leukaemia and Lymphoma Group conducted a prospective phase II study of pretransplant zoledronic acid (ZA) and individualised post-transplant ZA to prevent bone loss in alloSCT recipients. Patients received ZA 4 mg before conditioning. Administration of post-transplant ZA from days 100 to 365 post alloSCT was determined by a risk-adapted algorithm based on serial bone density assessments and glucocorticoid exposure. Of 82 patients enrolled, 70 were alive and without relapse at day 100. A single pretransplant dose of ZA prevented femoral neck bone loss at day 100 compared with baseline (mean change -2.6±4.6%). Using the risk-adapted protocol, 42 patients received ZA between days 100 and 365 post alloSCT, and this minimised bone loss at day 365 compared with pretransplant levels (mean change -2.9±5.3%). Femoral neck bone loss was significantly reduced in ZA-treated patients compared with historical untreated controls at days 100 and 365. This study demonstrates that a single dose of ZA pre-alloSCT prevents femoral neck bone loss at day 100 post alloSCT, and that a risk-adapted algorithm is able to guide ZA administration from days 100 to 365 post transplant and minimise further bone loss.

Organogenesis and foetal haemodynamics during the normal gestation of healthy black-rumped agoutis (Dasyprocta prymnolopha, Wagler, 1831) bred in captivity.

The objective of this study was to define the patterns of organogenesis and foetal haemodynamics during the normal gestation of healthy agoutis (Dasyprocta prymnolopha) kept in captivity. Thirty pregnant agoutis that ranged in size from small to medium and weighed between 2.5 and 3 kg underwent B-mode and Doppler ultrasonography for the biometric evaluation of the foetal organs. The foetal aortic blood flow proved to be predominantly systolic, and the measured flow velocity was 78.89 ± 2.95 cm/s, with a maximum pressure gradient of 2.12 ± 0.27 mmHg. The liver was characterized by its large volume, occupying the entire cranial aspect of the abdominal cavity, and it was associated cranially with the diaphragm and caudally with the stomach. The flow velocity in the portal vein was estimated to equal 12.17 ± 2.37 cm/s, with a resistivity index of 0.82 ± 0.05. The gallbladder was centrally located and protruded cranially towards the diaphragm. The spleen was visualized as an elongated structure with tapered cranial and caudal extremities, and the foetal kidneys were visualized bilaterally in the retroperitoneal region, with the right kidney positioned slightly more cranially than the left. The morphological characterization and hemodynamic analysis of the foetal organs of black-rumped agoutis via B-mode and Doppler ultrasonography allow determination of the vascular network and of reference values for the blood flow required for perfusing the anatomical elements essential for maintaining the viability of foetuses at different gestational ages.

Ornamental fishing in the region of Barcelos, Amazonas: socioeconomic description and scenario of activity in the view of piabeiros

Abstract The Negro river basin is considered the largest area of extractive of ornamental fish in Brazil. This area has fundamental importance for the populations from the Amazon. The present study aimed to describe socioeconomic profile of ornamental fishermen known as piabeiros in the Municipality of Barcelos, as well as the ornamental fishery, fisheries area, target species, environments, fishing techniques, equipment, capture techniques and main difficulties faced by the current activity. This study was carried out in municipality of Barcelos, through semi - structured interviews, with artisanal ornamental fishermen (N= 89). The main families of ornamental fish caught and traded were: Characidae, Lebiasinidae, Gasteropelecidae, Cichilidae, Anostomidae, Loricaridae, Potamotrygonidae and Gymnotidae. The main catchment areas were igarapés, lakes, flooded fields, beaches, river banks and igapó forest. Rapiché was the most used equipment in the fisheries both by the fishermen of the urban areas (43.81%) and rural (41.89%). Most of the fishermen are associated with the colony of fishermen of Barcelos (Z33). The data showed that the dynamics of ornamental fishing have changed in a short time and directly affected fishermen, in addition to the low age renewal with the participation of younger fishermen, threatening the transmission of ecological knowledge to future generations. As a result, the increase of the problems related to the productive chain and absence of public power to the activity, since ornamental fishing has already been treated as one of the main economic activities more important for the local communities and for the State of Amazonas.

Resumo A bacia do rio Negro é considerada a maior área de extrativismo de peixes ornamentais do Brasil. Esta área possui importância fundamental para as populações da Amazônia. O presente estudo objetivou descrever o perfil socioeconômico de pescadores conhecidos como piabeiros no município de Barcelos, bem como a pesca ornamental, área de pesca, espécies-alvo, ambientes, técnicas de pesca, equipamentos, técnicas de captura e dificuldades enfrentadas nesta atividade. O estudo foi realizado por meio de entrevistas semi - estruturadas, com pescadores artesanais ornamentais (N= 89). Os dados mostram que as famílias de peixes ornamentais capturados e comercializados foram: Characidae, Lebiasinidae, Gasteropelecidae, Cichilidae, Anostomidae, Loricaridae, Potamotrygonidae e Gymnotidae. As áreas de captura descritas foram igarapés, lagos, alagados, praias, margens de rios e floresta de igapó. O Rapiché foi o equipamento mais utilizado nas pescarias tanto pelos pescadores das áreas urbanas (43,81%) como rurais (41,89%). A maioria dos pescadores está associada à colônia de pescadores de Barcelos (Z33). Os dados coletados mostraram que a dinâmica da pesca ornamental mudou em pouco tempo e afetou diretamente os pescadores. Além da baixa taxa de renovação, com a participação de pescadores mais jovens, ameaçando a transmissão de conhecimento ecológico para as gerações futuras. Como resultado, notamos o aumento dos problemas relacionados à cadeia produtiva e a ausência de poder público na atividade. A pesca ornamental já foi tratada como uma das principais atividades econômicas para as comunidades locais e para o Estado do Amazonas.